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KMID : 0903619970380050575
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Journal of the Korean Society for Horticultural Science
1997 Volume.38 No. 5 p.575 ~ p.580
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Mass Propagation of Korean Native Styrax japonicus through Axillary Bud Culture
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Abstract
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To develop a mass propagation method of Korean native Styrax japonicas through an axillary bud culture for using as a woody landscape plant, about 2-3 ㎝ shoots induced from explant were subcultured to new media containing different growth regulators. Shoots multiplied most effectively on WPM containing 1.0 ㎎·L^(-1) zeatin, producing 1.7 shoots with a shoot length of 8.6 ㎝ per shoot explant. Multiple shoot development was most effective from the second node, while shoot tip was worst in shoot length and shoot number. Presence of leaves on cuttings did not exert an effect on multiple shoot development. Eighty percent of the shoots rooted after 20-30 days of culture with 1/2 GD control and the rooting rate decreased to 60%o with 1/2 GD + 0.5 ㎎·L^(-1) IBA treatment. Rooted plants were transplanted to a mixture of peatmoss and perlite (1:1, v/v) and grown under high humidity for 3 weeks in a greenhouse. After a hardening process with reduced humidity, 80% of the transplanted plants survived.
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